PoplarV1, Main, Exploration, bibRecord, 002389

A simple improved-throughput xylem protoplast system for studying wood formation.

Identifieur interne : 002389 ( Main/Exploration ); précédent : 002388; suivant : 002390

A simple improved-throughput xylem protoplast system for studying wood formation.

Auteurs : Ying-Chung Lin [États-Unis] ; Wei Li [États-Unis] ; Hao Chen [États-Unis] ; Quanzi Li [République populaire de Chine] ; Ying-Hsuan Sun [Taïwan] ; Rui Shi [États-Unis] ; Chien-Yuan Lin [États-Unis] ; Jack P. Wang [États-Unis] ; Hsi-Chuan Chen [États-Unis] ; Ling Chuang [États-Unis] ; Guan-Zheng Qu [République populaire de Chine] ; Ronald R. Sederoff [États-Unis] ; Vincent L. Chiang [États-Unis]

Source :

Nature protocols [ 1750-2799 ] ; 2014.

RBID : pubmed:25144270

Descripteurs français

KwdFr :
- Bois (croissance et développement), Bois (cytologie), Paroi cellulaire (métabolisme), Populus (croissance et développement), Populus (cytologie), Protoplastes (métabolisme), Protéines à fluorescence verte (MeSH), Régulation de l'expression des gènes végétaux (génétique), Réseaux de régulation génique (génétique), Transfection (méthodes), Xylème (composition chimique).
MESH :
- composition chimique : Xylème.
- croissance et développement : Bois, Populus.
- cytologie : Bois, Populus.
- génétique : Régulation de l'expression des gènes végétaux, Réseaux de régulation génique.
- métabolisme : Paroi cellulaire, Protoplastes.
- méthodes : Transfection.
- Protéines à fluorescence verte.

English descriptors

KwdEn :
- Cell Wall (metabolism), Gene Expression Regulation, Plant (genetics), Gene Regulatory Networks (genetics), Green Fluorescent Proteins (MeSH), Populus (cytology), Populus (growth & development), Protoplasts (metabolism), Transfection (methods), Wood (cytology), Wood (growth & development), Xylem (chemistry).
MESH :
- chemical : Green Fluorescent Proteins.
- chemistry : Xylem.
- cytology : Populus, Wood.
- genetics : Gene Expression Regulation, Plant, Gene Regulatory Networks.
- growth & development : Populus, Wood.
- metabolism : Cell Wall, Protoplasts.
- methods : Transfection.

Abstract

Isolated protoplasts serve as a transient expression system that is highly representative of stable transgenics in terms of transcriptome responses. They can also be used as a cellular system to study gene transactivation and nucleocytoplasmic protein trafficking. They are particularly useful for systems studies in which stable transgenics and mutants are unavailable. We present a protocol for the isolation and transfection of protoplasts from wood-forming tissue, the stem-differentiating xylem (SDX), in the model woody plant Populus trichocarpa. The method involves tissue preparation, digestion of SDX cell walls, protoplast isolation and DNA transfection. Our approach is markedly faster and provides better yields than previous protocols; small (milligrams)- to large (20 g)-scale SDX preparations can be achieved in ~60 s, with isolation of protoplasts and their subsequent transfection taking ~50 min. Up to ten different samples can be processed simultaneously in this time scale. Our protocol gives a high yield (~2.5 × 10(7) protoplasts per g of SDX) of protoplasts sharing 96% transcriptome identity with intact SDX.

DOI: 10.1038/nprot.2014.147
PubMed: 25144270

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 002039
to stream Main, to step Curation: 002039

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A simple improved-throughput xylem protoplast system for studying wood formation.</title>
<author><name sortKey="Lin, Ying Chung" sort="Lin, Ying Chung" uniqKey="Lin Y" first="Ying-Chung" last="Lin">Ying-Chung Lin</name>
<affiliation wicri:level="2"><nlm:affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
<affiliation wicri:level="2"><nlm:affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Hao" sort="Chen, Hao" uniqKey="Chen H" first="Hao" last="Chen">Hao Chen</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Quanzi" sort="Li, Quanzi" uniqKey="Li Q" first="Quanzi" last="Li">Quanzi Li</name>
<affiliation wicri:level="1"><nlm:affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA. [3] College of Forestry, Shandong Agricultural University, Taian, Shandong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA. [3] College of Forestry, Shandong Agricultural University, Taian, Shandong</wicri:regionArea>
<wicri:noRegion>Shandong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sun, Ying Hsuan" sort="Sun, Ying Hsuan" uniqKey="Sun Y" first="Ying-Hsuan" last="Sun">Ying-Hsuan Sun</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Forestry, National Chung Hsing University, Taichung, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Forestry, National Chung Hsing University, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Shi, Rui" sort="Shi, Rui" uniqKey="Shi R" first="Rui" last="Shi">Rui Shi</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lin, Chien Yuan" sort="Lin, Chien Yuan" uniqKey="Lin C" first="Chien-Yuan" last="Lin">Chien-Yuan Lin</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wang, Jack P" sort="Wang, Jack P" uniqKey="Wang J" first="Jack P" last="Wang">Jack P. Wang</name>
<affiliation wicri:level="2"><nlm:affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Hsi Chuan" sort="Chen, Hsi Chuan" uniqKey="Chen H" first="Hsi-Chuan" last="Chen">Hsi-Chuan Chen</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chuang, Ling" sort="Chuang, Ling" uniqKey="Chuang L" first="Ling" last="Chuang">Ling Chuang</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Qu, Guan Zheng" sort="Qu, Guan Zheng" uniqKey="Qu G" first="Guan-Zheng" last="Qu">Guan-Zheng Qu</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sederoff, Ronald R" sort="Sederoff, Ronald R" uniqKey="Sederoff R" first="Ronald R" last="Sederoff">Ronald R. Sederoff</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name>
<affiliation wicri:level="2"><nlm:affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA. [3] Department of Forest Biomaterials, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA. [3] Department of Forest Biomaterials, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2014">2014</date>
<idno type="RBID">pubmed:25144270</idno>
<idno type="pmid">25144270</idno>
<idno type="doi">10.1038/nprot.2014.147</idno>
<idno type="wicri:Area/Main/Corpus">002039</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002039</idno>
<idno type="wicri:Area/Main/Curation">002039</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002039</idno>
<idno type="wicri:Area/Main/Exploration">002039</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A simple improved-throughput xylem protoplast system for studying wood formation.</title>
<author><name sortKey="Lin, Ying Chung" sort="Lin, Ying Chung" uniqKey="Lin Y" first="Ying-Chung" last="Lin">Ying-Chung Lin</name>
<affiliation wicri:level="2"><nlm:affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
<affiliation wicri:level="2"><nlm:affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Hao" sort="Chen, Hao" uniqKey="Chen H" first="Hao" last="Chen">Hao Chen</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Quanzi" sort="Li, Quanzi" uniqKey="Li Q" first="Quanzi" last="Li">Quanzi Li</name>
<affiliation wicri:level="1"><nlm:affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA. [3] College of Forestry, Shandong Agricultural University, Taian, Shandong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA. [3] College of Forestry, Shandong Agricultural University, Taian, Shandong</wicri:regionArea>
<wicri:noRegion>Shandong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sun, Ying Hsuan" sort="Sun, Ying Hsuan" uniqKey="Sun Y" first="Ying-Hsuan" last="Sun">Ying-Hsuan Sun</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Forestry, National Chung Hsing University, Taichung, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Forestry, National Chung Hsing University, Taichung</wicri:regionArea>
<wicri:noRegion>Taichung</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Shi, Rui" sort="Shi, Rui" uniqKey="Shi R" first="Rui" last="Shi">Rui Shi</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lin, Chien Yuan" sort="Lin, Chien Yuan" uniqKey="Lin C" first="Chien-Yuan" last="Lin">Chien-Yuan Lin</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wang, Jack P" sort="Wang, Jack P" uniqKey="Wang J" first="Jack P" last="Wang">Jack P. Wang</name>
<affiliation wicri:level="2"><nlm:affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Hsi Chuan" sort="Chen, Hsi Chuan" uniqKey="Chen H" first="Hsi-Chuan" last="Chen">Hsi-Chuan Chen</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chuang, Ling" sort="Chuang, Ling" uniqKey="Chuang L" first="Ling" last="Chuang">Ling Chuang</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Qu, Guan Zheng" sort="Qu, Guan Zheng" uniqKey="Qu G" first="Guan-Zheng" last="Qu">Guan-Zheng Qu</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin</wicri:regionArea>
<wicri:noRegion>Harbin</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Sederoff, Ronald R" sort="Sederoff, Ronald R" uniqKey="Sederoff R" first="Ronald R" last="Sederoff">Ronald R. Sederoff</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name>
<affiliation wicri:level="2"><nlm:affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA. [3] Department of Forest Biomaterials, North Carolina State University, Raleigh, North Carolina, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA. [3] Department of Forest Biomaterials, North Carolina State University, Raleigh, North Carolina</wicri:regionArea>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Nature protocols</title>
<idno type="eISSN">1750-2799</idno>
<imprint><date when="2014" type="published">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (metabolism)</term>
<term>Gene Expression Regulation, Plant (genetics)</term>
<term>Gene Regulatory Networks (genetics)</term>
<term>Green Fluorescent Proteins (MeSH)</term>
<term>Populus (cytology)</term>
<term>Populus (growth & development)</term>
<term>Protoplasts (metabolism)</term>
<term>Transfection (methods)</term>
<term>Wood (cytology)</term>
<term>Wood (growth & development)</term>
<term>Xylem (chemistry)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Bois (croissance et développement)</term>
<term>Bois (cytologie)</term>
<term>Paroi cellulaire (métabolisme)</term>
<term>Populus (croissance et développement)</term>
<term>Populus (cytologie)</term>
<term>Protoplastes (métabolisme)</term>
<term>Protéines à fluorescence verte (MeSH)</term>
<term>Régulation de l'expression des gènes végétaux (génétique)</term>
<term>Réseaux de régulation génique (génétique)</term>
<term>Transfection (méthodes)</term>
<term>Xylème (composition chimique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Green Fluorescent Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Xylem</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Xylème</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Bois</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Bois</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Populus</term>
<term>Wood</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
<term>Gene Regulatory Networks</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term>
<term>Wood</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Régulation de l'expression des gènes végétaux</term>
<term>Réseaux de régulation génique</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term>
<term>Protoplasts</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Transfection</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Paroi cellulaire</term>
<term>Protoplastes</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Transfection</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Protéines à fluorescence verte</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Isolated protoplasts serve as a transient expression system that is highly representative of stable transgenics in terms of transcriptome responses. They can also be used as a cellular system to study gene transactivation and nucleocytoplasmic protein trafficking. They are particularly useful for systems studies in which stable transgenics and mutants are unavailable. We present a protocol for the isolation and transfection of protoplasts from wood-forming tissue, the stem-differentiating xylem (SDX), in the model woody plant Populus trichocarpa. The method involves tissue preparation, digestion of SDX cell walls, protoplast isolation and DNA transfection. Our approach is markedly faster and provides better yields than previous protocols; small (milligrams)- to large (20 g)-scale SDX preparations can be achieved in ~60 s, with isolation of protoplasts and their subsequent transfection taking ~50 min. Up to ten different samples can be processed simultaneously in this time scale. Our protocol gives a high yield (~2.5 × 10(7) protoplasts per g of SDX) of protoplasts sharing 96% transcriptome identity with intact SDX.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25144270</PMID>
<DateCompleted><Year>2015</Year>
<Month>03</Month>
<Day>30</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1750-2799</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>9</Volume>
<Issue>9</Issue>
<PubDate><Year>2014</Year>
<Month>Sep</Month>
</PubDate>
</JournalIssue>
<Title>Nature protocols</Title>
<ISOAbbreviation>Nat Protoc</ISOAbbreviation>
</Journal>
<ArticleTitle>A simple improved-throughput xylem protoplast system for studying wood formation.</ArticleTitle>
<Pagination><MedlinePgn>2194-205</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1038/nprot.2014.147</ELocationID>
<Abstract><AbstractText>Isolated protoplasts serve as a transient expression system that is highly representative of stable transgenics in terms of transcriptome responses. They can also be used as a cellular system to study gene transactivation and nucleocytoplasmic protein trafficking. They are particularly useful for systems studies in which stable transgenics and mutants are unavailable. We present a protocol for the isolation and transfection of protoplasts from wood-forming tissue, the stem-differentiating xylem (SDX), in the model woody plant Populus trichocarpa. The method involves tissue preparation, digestion of SDX cell walls, protoplast isolation and DNA transfection. Our approach is markedly faster and provides better yields than previous protocols; small (milligrams)- to large (20 g)-scale SDX preparations can be achieved in ~60 s, with isolation of protoplasts and their subsequent transfection taking ~50 min. Up to ten different samples can be processed simultaneously in this time scale. Our protocol gives a high yield (~2.5 × 10(7) protoplasts per g of SDX) of protoplasts sharing 96% transcriptome identity with intact SDX.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Ying-Chung</ForeName>
<Initials>YC</Initials>
<AffiliationInfo><Affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Wei</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Hao</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Quanzi</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA. [3] College of Forestry, Shandong Agricultural University, Taian, Shandong, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sun</LastName>
<ForeName>Ying-Hsuan</ForeName>
<Initials>YH</Initials>
<AffiliationInfo><Affiliation>Department of Forestry, National Chung Hsing University, Taichung, Taiwan.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shi</LastName>
<ForeName>Rui</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Chien-Yuan</ForeName>
<Initials>CY</Initials>
<AffiliationInfo><Affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Jack P</ForeName>
<Initials>JP</Initials>
<AffiliationInfo><Affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Hsi-Chuan</ForeName>
<Initials>HC</Initials>
<AffiliationInfo><Affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chuang</LastName>
<ForeName>Ling</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Qu</LastName>
<ForeName>Guan-Zheng</ForeName>
<Initials>GZ</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sederoff</LastName>
<ForeName>Ronald R</ForeName>
<Initials>RR</Initials>
<AffiliationInfo><Affiliation>Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chiang</LastName>
<ForeName>Vincent L</ForeName>
<Initials>VL</Initials>
<AffiliationInfo><Affiliation>1] State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China. [2] Department of Forestry and Environmental Resources, Forest Biotechnology Group, North Carolina State University, Raleigh, North Carolina, USA. [3] Department of Forest Biomaterials, North Carolina State University, Raleigh, North Carolina, USA.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2014</Year>
<Month>08</Month>
<Day>21</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Nat Protoc</MedlineTA>
<NlmUniqueID>101284307</NlmUniqueID>
<ISSNLinking>1750-2799</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>147336-22-9</RegistryNumber>
<NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053263" MajorTopicYN="N">Gene Regulatory Networks</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011523" MajorTopicYN="N">Protoplasts</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014162" MajorTopicYN="N">Transfection</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year>
<Month>8</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2014</Year>
<Month>8</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2015</Year>
<Month>3</Month>
<Day>31</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">25144270</ArticleId>
<ArticleId IdType="pii">nprot.2014.147</ArticleId>
<ArticleId IdType="doi">10.1038/nprot.2014.147</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Nat Protoc. 2007;2(7):1565-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17585298</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Biotechnol. 2000 Jun;11(3):298-302</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10851143</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2007 Sep;51(6):1126-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17666025</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2006 Nov;47(11):1582-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17018558</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2008;59(3):681-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18281718</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Methods. 2009 Nov 24;5:16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19930690</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2007;2(10):2348-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17947977</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2001 Dec;127(4):1466-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11743090</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Jun 25;110(26):10848-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23754401</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2013 Nov;25(11):4282</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24285792</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2009 Dec 3;462(7273):660-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19924127</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Mol Biol. 2010 Nov;74(4-5):367-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20803312</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14699-704</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22915581</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 2002 Jun;20(6):557-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12042854</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2013 Dec 20;342(6165):1241089</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24357323</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 2011 Apr;30(4):473-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21103876</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Methods. 2010 Jan 14;6(1):1</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20180960</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2013 Nov;25(11):4324-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24280390</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2006 Jan 27;311(5760):484-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16439654</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 May 30;497(7451):579-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23698360</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):21253-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22160716</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Mol Biol. 2006 Jan 31;7:3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16448564</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1976 Feb 20;191(4228):773-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17754175</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Methods. 2011 Sep 30;7(1):30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21961694</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2006 Sep 15;313(5793):1596-604</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16973872</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2006 Jul;47(7):905-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16720648</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2013 Jul;25(7):2444-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23903317</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2011 Jun;156(2):474-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21454800</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2001 Jul;13(7):1567-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11449052</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2012;7(9):e44908</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23028673</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 Dec 12;302(5652):1956-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14671301</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>In Vitro Cell Dev Biol Plant. 2009 Dec;45(6):619-629</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19936031</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2009 Feb;149(2):1141-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19028881</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4939-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12668766</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2009 Apr;58(2):260-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19175765</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
<li>Taïwan</li>
<li>États-Unis</li>
</country>
<region><li>Caroline du Nord</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Lin, Ying Chung" sort="Lin, Ying Chung" uniqKey="Lin Y" first="Ying-Chung" last="Lin">Ying-Chung Lin</name>
</region>
<name sortKey="Chen, Hao" sort="Chen, Hao" uniqKey="Chen H" first="Hao" last="Chen">Hao Chen</name>
<name sortKey="Chen, Hsi Chuan" sort="Chen, Hsi Chuan" uniqKey="Chen H" first="Hsi-Chuan" last="Chen">Hsi-Chuan Chen</name>
<name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name>
<name sortKey="Chuang, Ling" sort="Chuang, Ling" uniqKey="Chuang L" first="Ling" last="Chuang">Ling Chuang</name>
<name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
<name sortKey="Lin, Chien Yuan" sort="Lin, Chien Yuan" uniqKey="Lin C" first="Chien-Yuan" last="Lin">Chien-Yuan Lin</name>
<name sortKey="Sederoff, Ronald R" sort="Sederoff, Ronald R" uniqKey="Sederoff R" first="Ronald R" last="Sederoff">Ronald R. Sederoff</name>
<name sortKey="Shi, Rui" sort="Shi, Rui" uniqKey="Shi R" first="Rui" last="Shi">Rui Shi</name>
<name sortKey="Wang, Jack P" sort="Wang, Jack P" uniqKey="Wang J" first="Jack P" last="Wang">Jack P. Wang</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Li, Quanzi" sort="Li, Quanzi" uniqKey="Li Q" first="Quanzi" last="Li">Quanzi Li</name>
</noRegion>
<name sortKey="Qu, Guan Zheng" sort="Qu, Guan Zheng" uniqKey="Qu G" first="Guan-Zheng" last="Qu">Guan-Zheng Qu</name>
</country>
<country name="Taïwan"><noRegion><name sortKey="Sun, Ying Hsuan" sort="Sun, Ying Hsuan" uniqKey="Sun Y" first="Ying-Hsuan" last="Sun">Ying-Hsuan Sun</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002389 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002389 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:25144270
   |texte=   A simple improved-throughput xylem protoplast system for studying wood formation.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:25144270" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 12:07:19 2020. Site generation: Wed Nov 18 12:16:31 2020

	Serveur d'exploration sur le peuplier
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le peuplier

A simple improved-throughput xylem protoplast system for studying wood formation.

A simple improved-throughput xylem protoplast system for studying wood formation.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki